FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope

Abstract

We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.

DOI
10.1364/MICROSCOPY.2020.MTu4A.7
Year